Molecular Research on Vector-Borne Diseases of Medical Interest: From Bench to Application 2.0

Infectious diseases caused by parasites (malaria, leishmaniasis, trypanosomiasis, filariasis…), viruses (chikungunya, dengue, phlebovirus, etc [...].

the formation of brain cysts in T. gondii-infected mice. The JF363 compound docking in the five annotated ME49 T. gondii HDAC-active sites displays a similar binding mode in the five paralogous structures and a similar micromolar range prediction of affinities. These results will support future development aimed at treating acute and chronic toxoplasmosis and developing anti-Plasmodium therapeutic interventions.
Leishmanolysin, or promastigote protease (PSP) or gp63, is an abundant parasite virulence factor surface glycoprotein of Leishmania promastigotes. The metalloprotease activity of gp63 is inhibited powerfully by metal chelators. Here, the three-dimensional structure of gp63 and its active site was modelized and used as a template to search for possible inhibitors in the literature and molecular databases. Molecular docking simulations of the generated protein-ligand complexes were computed. The individual energy of gp63 amino acids interacting with its ligands was quantified by ab initio calculations using Molecular Fractions with Conjugated Caps (MFCC). The MFCC analysis of each candidate allowed quantum balance calculations for protein interaction. A compound (L1), a 7-hydroxycoumarin derivative, obtains the best energy quantum balance result (−2 eV), followed by DETC, doxycycline, and 4-terpineol. In vitro experiments confirmed L1 antileishmanial activity against L. amazonensis promastigotes, low cytotoxicity against mammalian RAW and 3T3 cell lines, and a selectivity index of 149.19 and 380.64 µM, respectively. The L1 compound induced promastigote death by necrosis, with disruption in membrane integrity further documented by Spectroscopy Dispersion analysis. The L1 would be an effective compound against L. amazonensis, the etiologic agent of diffuse cutaneous leishmaniasis.
Leishmania tarentolae, a non-human pathogenic trypanosomatid, is frequently used as a heterologous protein expression system. Nevertheless, it can help delineate the underlying virulence and pathogenic mechanisms involved in human and animal leishmaniases. The repertoire of Leishmaniolysin genes in the genome of L. tarentolae was investigated by Ennes-Vidal et al. [12], and a total of 61 leishmanolysin sequences were retrieved. These are phylogenetically more related to the L. major leishmaniolysin than the L. braziliensis and L. martiniquensis. Surprisingly no proteolytic activity is detected. The 3D homology models based on the crystallographic structure of L. major ortholog were built with three L. tarentolae gp63 sequences. Molecular dynamics simulations disclosed a lower electrostatic negative potential than the L. major template for the three models constructed in silico. The L. major LmjF.10.0460 and the L. tarentolae LtaP10.0650 leishmanolysins genes were then cloned into the pLEXSY vector and transfected into L. tarentolae to allow their expression. The authors observed that L. tarentolae leishmanolysins harbor a weak enzymatic activity, three times less than L. major ones. This suggests that the lower electrostatic negative potential supported by L. tarentolae leishmanolysin contributes to the reduced proteolytic activity detected in this parasite species.
Characterizing enzymes allowing fatty (FA) acid synthesis by Leishmania opens perspectives on understanding their role in parasite biology and development. The review proposed by Leroux et al. [13] provides clues on FA composition profiles and the metabolism of the major lipids and phospholipids classes in Leishmania species responsible for cutaneous or visceral diseases. In L. major, T. cruzi, and T. brucei, in human and animal trypanosomatid pathogens, several enzymes involved in de novo FA synthesis have emerged as potential drug targets. Drug resistance to first-line antileishmanial compounds is documented [14][15][16][17]; but their implication in therapeutic failure is debated [18]. Changes in FA compositions are observed in several Leishmania species upon exposure to antileishmanial drugs and resistant Leishmania isolates. These changes are proposed as putative mechanisms for drug toxicity or drug resistance. The conversion of polyunsaturated fatty acids and their metabolites into inflammatory mediators and oxygenated metabolites, acting on metacyclogenesis and parasite infectivity, opens perspectives on understanding the impact of lipid status on the development of leishmaniasis. Therefore, fatty acids and their metabolism may offer new therapeutic targets but would also be interesting for innovative nutritional interventions.

A second set of papers deals with diagnostics issues for Rocio virus and Trypanosomatidae infections.
Rocio virus infection is a neglected threat transmitted by mosquitoes. New immune inputs are required for serological diagnosis and epidemiological surveillance in lowresource settings. An in silico approach identifies a specific antigenic peptide (p_ROCV2) in the NS1 Rocio virus protein, which is predicted to be stable and exposed on the virus surface. It demonstrates the essential properties required to interact with antibodies. These findings provide insights needed to push up the development of a diagnostic platform and investigate therapeutic alternatives.
Detection and diagnosis of the human and animal infections caused by Trypanosomatidae family parasites remain challenging [19][20][21]. The isothermal amplification of nucleic acids has the potential to be applied in resource-limited areas for the detection of infectious agents, as it does not require complex nucleic purification steps or specific and expensive equipment and reagents to perform the reaction and read the results. Since human and animal infections by pathogens of the Trypanosomatidae family occur mainly in resource-limited areas with scant health infrastructures and personnel, in conjunction with non-invasive biological biopsy [8], these methodologies would therefore hold great promise. Sereno et al. [22] propose a critical literature review on applying isothermal nucleic acid amplification to detect Trypanosoma and Leishmania infections. The authors highlight gaps and propose ways to fill them to translate these powerful technologies into real-world field applications for a neglected human and animal diseases caused by Trypanosomatidae.
In the northern hemisphere, the ectoparasite Ixodes ricinus is a vector for tick-borne diseases (TBD) such as rickettsiosis, Lyme borreliosis, human granulocytic anaplasmosis, or the tick-borne encephalitis virus [23,24]. Owing to climate change, a temperature rise is anticipated following in the coming years. That would increase tick activity and population, spreading TBD. Consequently, it is critical to understand how microbial tick communities contribute to TBD's fitness and occurrence. Using 16S rRNA gene amplicon sequencing, Weisinger et al. [25] analyzed bacterial taxon diversity in various tick organs and tissues (midgut, salivary glands) and the residual tick material on whole Ixodes ricinus. They use a DNA extraction protocol, newly developed for tick samples, and a self-designed mock community. This new approach dissecting ticks and isolating tissue-extracting tick DNA using an automated DNA extraction method and employing a tick-specific mock community-allows for the effective identification of tick-specific pathogens and endosymbionts. This approach and the attendant recommendations for using a tick-specific mock community will enable the observation of pathogens' prevalences and abundances in tick populations. It might be used to assess the danger of TBD infections in particular forest and park areas, thus allowing hazard assessment.
Schistosomiasis, also known as snail fever or bilharzia, is a parasitic disease caused by Schistosoma, a trematode flatworm. The World Health Organization considers it the second most prevalent parasitic disease after malaria, affecting more than 230 million people in over 70 countries. Infection occurs via various activities ranging from agricultural, domestic, and occupational activities to recreational activities, where the freshwater snails, Biomphalaria, release Schistosoma cercariae larvae that penetrate the skin of humans when exposed to water. Au et al. [26] review the latest molecular studies on the snail Biomphalaria, its ecology, evolution, and immune response. It proposes genomics as a foundation by which to understand and control this disease vector and thus, schistosomiasis transmission.
This Special Issue has provided welcome opportunities to focus on some neglected vector-borne pathologies (e.g., leishmaniasis, schistosomiasis . . . ) from the understudied and underreported Rocio virus to various tick-borne pathogens. It will help delineate new diagnostic tools and strategies, therapeutic approaches, and future drug targets. It will also emphasize the interest of genomics to question the emergence and spread of vector-borne infections.